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CELLSCREENTitle of the Sub-Project: CELLSCREEN - Spatially defined addressing of cells to surfaces Description: Tissues and organs consist of different cell types that communicate with each other and the surrounding extracellular matrix (ECM). Crucial for the maintenance and repair of tissues is a well-defined 3D arrangement of the different cells that e.g. decides on the spatial location of stem cell niches. This is exemplified by the structure of the skin. At the interface between epidermis and sclera, ECM material is deposited. Furthermore, the stem cells responsible for continuous regeneration of the epidermis are located at that very interface. As these cells divide, they depart from the sclera / ECM and concomitantly loose their stem cell features. The signals responsible for this change of character are poorly understood, which is one of the main obstacles in Tissue Engineering that aims e.g. to assist in the repair of dysfunctional organs and tissues. Cells within organs or stem cell niches measure their surroundings through cell-cell or cell-ECM interactions. Thus, the 3D location decides if a cell e.g. is to stay committed as a stem cell or instead differentiates into an epidermal cell. One way to understand the nature of the relevant signals is to investigate the influence of different spatial arrangements of different cell types on the secretion of ECM proteins. Therefore, our objective is to variably pattern a 2D surface with small peptides that distinctively engage cell adhesion receptors from different cell types. Next, the optimal (2D-) spatial arrangement for ECM secretion will be tested simply by capturing and staining ECM material with suitable monoclonal antibodies. However, crucial for the success of this and similar investigations is the availability of peptides other than the integrin-engaging RGD-tripeptides that distinctively engage different cell types. Therefore, our objective is to screen a library of 130,000 small peptides bound to a solid support to detect the ones that engage cell receptors and thus hold the promise to spatially arrange different cell types onto a solid support. In the course of the project, all partners profit from their complimentary know-how. Therefore, the collaboration is considered to be sustainable in the future. A successful project would generate results that enable the participants to publish their technology, expand it to other fields, and promote translational research. One of the main strategic goals of CELLSCREEN is also the promotion of spin-offs and other SMEs. To reach this goal the commercialisation of the peptide laser technology will be done by a regional SME, the read-out systems will be commercialised by UAR. Project Partners: - German Cancer Research Centre (DKFZ) (D) - Fraunhofer Institute for Manufacturing Engineering and Automation IPA (D) - Upper Austrian Research GmbH/Centre for Biomedical Nanotechnology (A) Contact: For further questions please contact: PD Dr. Frank Breitling DKFZ (German Cancer Research Centre) Im Neuenheimer Feld 280 69120 Heidelberg GERMANY Tel.: +49 6221 424744 Fax: +49 6221 421744 E-mail: f.breitling@dkfz.de  Project Sheet CELLSCREEN
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